Spermidine supplier specification
Nutrition BioTech is a bulk spermidine ingredient supplier and manufacturer, supplying Autophito™ spermidine at 98% minimum by HPLC, available as the free base (CAS 124-20-9) or as the trihydrochloride salt (CAS 334-50-9), for B2B nutraceutical and longevity-category formulation.
Every lot ships with a Certificate of Analysis stating identity, HPLC assay and the assay basis, from an ISO 22000 and FSSC 22000 certified quality system. Samples, specifications and COAs are available on request for formulation trials.
| Ingredient name | Spermidine 98% (Autophito™) |
|---|---|
| Synonyms | N-(3-aminopropyl)butane-1,4-diamine; 4-aminobutyl-1,3-propanediamine; spermidine trihydrochloride (salt form) |
| CAS number | 124-20-9 (free base); 334-50-9 (trihydrochloride) |
| Machine-readable identifiers | CAS 124-20-9 — InChIKey ATHGHQPFGPMSJY-UHFFFAOYSA-N · PubChem CID 1102 CAS 334-50-9 — InChIKey LCNBIHVSOPXFMR-UHFFFAOYSA-N · PubChem CID 9539 |
| Molecular formula | C7H19N3 (free base); C7H19N3 · 3HCl (trihydrochloride) |
| Molecular weight | 145.25 g/mol (free base); 254.63 g/mol (trihydrochloride) |
| Assay / purity | 98% minimum by HPLC. Free base or trihydrochloride salt is confirmed on the specification sheet before sampling; other grades available on request. |
| Production route | Manufactured material. The salt form and production route for the supplied grade are stated on its specification sheet. |
| Appearance | Trihydrochloride salt: white to off-white crystalline powder. Free base: colourless to pale yellow liquid or low-melting solid. |
| Solubility | Soluble in water; the free base is hygroscopic and moisture-sensitive. |
| Typical applications | Low-dose capsules and tablets, sachets, liposomal concepts and multi-ingredient longevity blends for B2B dietary-supplement programs. |
| Documentation | Download public specification sheet Public sheet prepared from the current product-page specification block; not a batch-specific Certificate of Analysis. Lot-specific COA and supporting records are available for qualified buyer review when appropriate. |
| Quality system | ISO 22000:2018, FSSC 22000 and HACCP based quality system, GMP-aligned manufacturing; certificates available on request. |
| Supplier | Nutrition BioTech, 1601 W. Mission Blvd, Suite 103 (DL#29), Pomona, CA 91766, United States |
Spermidine Trihydrochloride Specification Limits And Analytical Methods
Every specification below is the limit carried on our published product specification sheet, with the analytical method stated for each item.
| Test item | Specification | Method |
|---|---|---|
| Spermidine trihydrochloride (assay) | 95.0–105.0% | NMR |
| Character | Solid white powder, characteristic odour and taste, no visible impurities | Organoleptic |
| Moisture | NMT 1.0% | Ch.P <0832> |
| Residue on ignition | NMT 0.2% | Ch.P <0841> |
| Heavy metals (total) | NMT 10 ppm | Ch.P <0821> |
| Lead | NMT 0.5 ppm | Ch.P <2321> |
| Arsenic | NMT 0.5 ppm | Ch.P <2321> |
| Mercury | NMT 0.1 ppm | Ch.P <2321> |
| Cadmium | NMT 0.5 ppm | Ch.P <2321> |
| Total aerobic bacterial count | NMT 1,000 cfu/g | Ch.P <1105> |
| Yeast and mould | NMT 100 cfu/g | Ch.P <1105> |
| E. coli | Absent | Ch.P <1106> |
| Salmonella | Absent | Ch.P <1106> |
- Assay is quantified by NMR rather than a routine HPLC-only line. For a polyamine without a strong chromophore, qNMR is a direct quantitative route and avoids derivatization-dependent ambiguity.
- Elemental limits are tight: Pb, As and Cd each NMT 0.5 ppm; Hg NMT 0.1 ppm.
- This specification uses Ch.P methods as carried on the published product specification sheet.
Method and limit block for buyer QA review. Batch-specific results, lot numbers, production dates and expiry dates are not published here.
Spermidine 98% (Autophito™)
High-purity spermidine for autophagy-positioned and cellular renewal formulas.
Why brands ask for it
Autophito™ Spermidine 98% is positioned for premium longevity formulas focused on autophagy and cellular renewal. It suits low-dose capsules, liposomal concepts and multi-ingredient longevity stacks where purity and documentation matter.
Finished-product fit
Autophagy support, cellular renewal, healthy aging, fasting-mimic longevity routines. The strongest conversations start with format, serving size, documentation, and how the material behaves in the intended formula.
What makes the sample useful
- High purity 98%
- Low-dose premium active
- Strong fit with NAD, fisetin, quercetin and resveratrol
Before the sample ships
- Confirm source, identity, and supporting documentation.
- Confirm free base or salt.
- Review serving-size logic and label-space fit.
- Check taste, odor, and sensory fit before formula lock.
- Review stability expectations and storage conditions for the finished format.
File Package
Ask for the material files that match your market, dosage form, and internal review process.
Available documents may include: COA / Specification / Allergen Statement / GMO Statement / Vegan Statement / Heavy Metals / Microbial Limits / Manufacturing Flow Chart / Residual Solvent / Country of Origin / Composition Statement.
Regulatory & Facility Support
Regulatory review notes for the target market can be provided. Nutrition BioTech does not hold and does not claim any GRAS notification or NDI notification for this material.
Facility and quality system support follows the same certificate set named in the specification table: ISO 22000:2018, FSSC 22000, HACCP and GMP. The holding entity for each certificate is named on the certificate and confirmed under buyer review.
Regulatory status of Spermidine
The entries below summarise publicly available regulatory documents and are provided as context for buyers. They are not claims about Nutrition BioTech material, and no health outcome is claimed or implied for any finished product. Authorisation is specific to the source, form and conditions of use described in each instrument. Buyers are responsible for confirming the status of the ingredient in their own market and for the claims they make on their own labels. Last reviewed: 3 August 2026.
What the EU novel food authorisation actually covers
The novel food entered in the Union list is “spermidine-rich wheat germ extract (Triticum aestivum)”. Commission Implementing Regulation (EU) 2020/443 of 25 March 2020 authorised a change to its specifications under Regulation (EU) 2015/2283 and amended Commission Implementing Regulation (EU) 2017/2470. The authorisation is tied to that extract and its specifications; it does not extend to isolated or synthetically produced spermidine or to spermidine salts.
Commission Implementing Regulation (EU) 2020/443, 25 March 2020 — source
Target population in the conditions of use
The conditions of use recorded for spermidine-rich wheat germ extract in the Union list specify the adult population excluding pregnant and lactating women.
Union list entry, Commission Implementing Regulation (EU) 2017/2470 as amended — source
Why the form supplied matters for market access
Because novel food authorisation is specific to the material and specification described in the instrument, the production route and salt form of a spermidine material determine which authorisation, if any, applies. Buyers should confirm the regulatory position of the specific form they intend to use in each target market before formulating.
Regulation (EU) 2015/2283 on novel foods — source
Specification FAQ
What is the CAS number for Spermidine?
The CAS number for Spermidine is 124-20-9 (free base); 334-50-9 (trihydrochloride). The molecular formula is C7H19N3 (free base); C7H19N3 · 3HCl (trihydrochloride) and the molecular weight is 145.25 g/mol (free base); 254.63 g/mol (trihydrochloride). Machine-readable identifiers: CAS 124-20-9 — InChIKey ATHGHQPFGPMSJY-UHFFFAOYSA-N · PubChem CID 1102CAS 334-50-9 — InChIKey LCNBIHVSOPXFMR-UHFFFAOYSA-N · PubChem CID 9539.
What purity is Spermidine supplied at, and by which method?
98% minimum by HPLC. Free base or trihydrochloride salt is confirmed on the specification sheet before sampling; other grades available on request.
Is Spermidine soluble in water?
Soluble in water; the free base is hygroscopic and moisture-sensitive.
Published research context
Spermidine has a long peer-reviewed literature. The studies below are provided as technical context for formulators evaluating this raw material. These entries summarise what the cited publications report. They are not claims about Nutrition BioTech material, and no health, therapeutic or cosmetic outcome is claimed or implied for any finished product. Buyers are responsible for the claims they make on their own labels and for confirming what is permitted in their market.
- Fully validated dansyl chloride HPLC assayValidated a high-performance liquid chromatography method with photodiode array detection for spermidine, using pre-column derivatisation with dansyl chloride, separation on a core-shell C18 column and detection at 254 nm. The method was validated according to United States Food and Drug Administration guidance for bioanalytical method validation with regard to selectivity, precision, accuracy, recovery, linearity, limits of detection and quantitation, and standard-solution, short-term, long-term, freeze-thaw and benchtop stability. Detector response was reported as linear from 0.002 to 8 mg/L with a correlation coefficient greater than 0.999, with a limit of detection of 1 ug/L and a limit of quantitation of 2 ug/L.Esatbeyoglu T, Ehmer A, Chaize D, Rimbach G. J Agric Food Chem. 2016;64(10):2105-2111. PMID 26915410. DOI 10.1021/acs.jafc.6b00078. source
- Sample clean-up before derivatisationDescribed an improved dansyl derivatisation procedure in which amines were extracted with 1 percent trichloroacetic acid, cleaned up on a polymer-based strong cation-exchange mini-column and eluted with 1 mol/L potassium carbonate before dansylation and HPLC. The reported limit of quantitation for spermidine was 0.8 ug/g, and average recoveries of the five amines studied across nine foods exceeded 80 percent.Handa A, Kawanabe H, Ibe A. Shokuhin Eiseigaku Zasshi. 2017;58(3):149-154. PMID 28690305. DOI 10.3358/shokueishi.58.149. source
- Encapsulation efficiency, water activity and residual moisture in a dried carrier systemPrepared water-in-oil-in-water emulsions and freeze-dried microcapsules using spermidine recovered from beech mushroom cooking liquor as the core material in a soy protein isolate dextran and sodium alginate system. Encapsulation efficiency of 92.90 to 99.76 percent and retention efficiency of 92.47 to 96.87 percent were reported for the emulsions, and encapsulation efficiency of 56.44 to 98.13 percent, retention efficiency of 70.21 to 89.12 percent, water activity of 0.2139 to 0.2279 and moisture content of 2.14 to 2.88 percent were reported for the freeze-dried microcapsules. The authors noted that direct exposure to spermidine can contribute bitterness.Xia C, Zhang M. Foods. 2026;15(10):1734. PMID 42195937. DOI 10.3390/foods15101734. source
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