Ergothioneine 99% supplier specification
Nutrition BioTech is a bulk L-ergothioneine ingredient supplier and manufacturer, supplying L-(+)-ergothioneine (CAS 497-30-3) at 99% minimum by HPLC, produced by microbial fermentation, for B2B nutraceutical and cosmetic formulation.
Ergothioneine is a low-dose material, so identity, assay basis and serving-size arithmetic matter more than tonnage. Both fermentation-derived and chemically synthesised ergothioneine exist on the market; the production route for the supplied lot is stated on the specification sheet, since route affects regulatory filings in several markets.
| Ingredient name | Ergothioneine 99% (L-ergothioneine) |
|---|---|
| Synonyms | L-(+)-Ergothioneine; EGT; 2-mercaptohistidine trimethylbetaine; thiolhistidine betaine |
| CAS number | 497-30-3 |
| Machine-readable identifiers | InChIKey SSISHJJTAXXQAX-ZETCQYMHSA-N · PubChem CID 5351619 |
| Molecular formula | C9H15N3O2S |
| Molecular weight | 229.30 g/mol |
| Assay / purity | 99% minimum by HPLC, stated on the Certificate of Analysis for each lot. |
| Source / production route | Microbial fermentation followed by purification. The organism class and route for the supplied lot are stated on the specification sheet; synthetic grades can be quoted separately where a buyer specifies them. |
| Appearance | White to off-white crystalline powder |
| Solubility | Soluble in water; sparingly soluble in ethanol. Stable across the pH range used in most beverage and capsule formats, with the thione group giving good oxidative stability relative to thiol antioxidants. |
| Typical applications | Capsules, tablets, sachets, beauty-from-within beverages and topical cosmetic bases for B2B manufacturing; used at low inclusion levels, commonly alongside NAD+ precursors, glutathione and vitamin C |
| Documentation | Download public specification sheet Public sheet prepared from the current product-page specification block; not a batch-specific Certificate of Analysis. Lot-specific COA and supporting records are available for qualified buyer review when appropriate. |
| Quality system | ISO 22000:2018, FSSC 22000, HACCP and GMP (certificates provided on request) |
| Supplier | Nutrition BioTech, 1601 W. Mission Blvd, Suite 103 (DL#29), Pomona, CA 91766, United States |
Ergothioneine 99%
High-purity ergothioneine for premium skin longevity and cellular antioxidant formulas.
Why brands ask for it
Ergothioneine 99% is a premium low-dose antioxidant ingredient for beauty-from-within and longevity formulas. It fits skin aging, cellular protection and high-end healthy-aging concepts where purity and documentation drive buyer confidence.
Finished-product fit
Skin longevity, beauty from within, cellular protection, premium antioxidant. The strongest conversations start with format, serving size, documentation, and how the material behaves in the intended formula.
What makes the sample useful
- High-purity 99%
- Low-dose premium ingredient
- Strong fit for skin-aging and longevity stacks
Before the sample ships
- Confirm source, identity, and supporting documentation.
- Confirm it fermentation.
- Review serving-size logic and label-space fit.
- Review stability expectations and storage conditions for the finished format.
- Confirm it worth premium price.
File Package
Ask for the material files that match your market, dosage form, and internal review process.
Available documents may include: COA / Specification / Allergen Statement / GMO Statement / Vegan Statement / Heavy Metals / Microbial Limits / Manufacturing Flow Chart / Residual Solvent / Country of Origin / Composition Statement.
Regulatory & Facility Support
Regulatory review notes for the target market can be provided. Nutrition BioTech does not hold and does not claim any GRAS notification or NDI notification for this material.
Facility and quality system support follows the same certificate set named in the specification table: ISO 22000:2018, FSSC 22000, HACCP and GMP. The holding entity for each certificate is named on the certificate and confirmed under buyer review.
Regulatory status of L-Ergothioneine
The entries below summarise publicly available regulatory documents and are provided as context for buyers. They are not claims about Nutrition BioTech material, and no health outcome is claimed or implied for any finished product. Authorisation is specific to the source, form and conditions of use described in each instrument. Buyers are responsible for confirming the status of the ingredient in their own market and for the claims they make on their own labels. Last reviewed: 3 August 2026.
Authorised as a novel food in the European Union
Commission Implementing Decision (EU) 2017/1281 of 13 July 2017 authorised the placing on the market of L-ergothioneine as a novel food ingredient under Regulation (EC) No 258/97.
Commission Implementing Decision (EU) 2017/1281, 13 July 2017 — source
Extension of use and entry in the Union list
Commission Implementing Regulation (EU) 2018/462 of 20 March 2018 authorised an extension of use of L-ergothioneine as a novel food under Regulation (EU) 2015/2283 and amended Commission Implementing Regulation (EU) 2017/2470. The entry in the Union list carries the conditions of use and labelling requirements set out in the Annex to that Regulation, and the designation on the labelling of foods containing it is “L-ergothioneine”.
Commission Implementing Regulation (EU) 2018/462, 20 March 2018 — source
Where the binding conditions of use are set out
The Union list of novel foods authorised for placing on the market in the Union is set out in the Annex to Commission Implementing Regulation (EU) 2017/2470. Permitted food categories, maximum levels and specifications are defined there and should be checked against the intended food category and target population before formulating.
Commission Implementing Regulation (EU) 2017/2470, 20 December 2017 — source
Specification FAQ
What is the CAS number for Ergothioneine?
The CAS number for Ergothioneine is 497-30-3. The molecular formula is C9H15N3O2S and the molecular weight is 229.30 g/mol. Machine-readable identifiers: InChIKey SSISHJJTAXXQAX-ZETCQYMHSA-N · PubChem CID 5351619.
What purity is Ergothioneine supplied at, and by which method?
99% minimum by HPLC, stated on the Certificate of Analysis for each lot.
Is Ergothioneine soluble in water?
Soluble in water; sparingly soluble in ethanol. Stable across the pH range used in most beverage and capsule formats, with the thione group giving good oxidative stability relative to thiol antioxidants.
How is Ergothioneine produced and where does it come from?
Microbial fermentation followed by purification. The organism class and route for the supplied lot are stated on the specification sheet; synthetic grades can be quoted separately where a buyer specifies them.
Published research context
L-Ergothioneine has a long peer-reviewed literature. The studies below are provided as technical context for formulators evaluating this raw material. These entries summarise what the cited publications report. They are not claims about Nutrition BioTech material, and no health, therapeutic or cosmetic outcome is claimed or implied for any finished product. Buyers are responsible for the claims they make on their own labels and for confirming what is permitted in their market.
- Validated HILIC assay in fermentation brothEstablished a hydrophilic interaction liquid chromatography method for ergothioneine in fermentation broth, using a HILIC column with an isocratic mobile phase of acetonitrile and 20 mmol/L ammonium acetate (85:15, v/v) adjusted to pH 6.0 with acetic acid, UV detection at 254 nm, a flow rate of 1.0 mL/min and a column temperature of 40 degrees Celsius. Linearity was reported over 5 to 400 mg/L with a correlation coefficient of 0.9999, recoveries of 99.2 to 100.8 percent, and relative standard deviations for repeatability, intermediate precision and stability of 1.47, 1.03 and 1.66 percent.Liu Q, Zhang W, Wang H, Li Y. J Chromatogr Sci. 2016;54(6):934-938. PMID 26921894. DOI 10.1093/chromsci/bmw023. source
- Thiol-specific post-column detection as an alternative to mass spectrometryDeveloped a reversed-phase separation with a post-column reaction between 2,2-dipyridyl disulphide and ergothioneine, detecting the resulting 2-thiopyridone at 343 nm. The reported limit of quantitation was 1.41 ppm, recoveries were 97.5 to 100.0 percent, and the coefficient of variation for reproducibility was within 6 percent. The authors reported that the simpler sample preparation made the technique more rapid than the liquid chromatography mass spectrometry methods it was compared with.Nguyen TH, Giri A, Ohshima T. Food Chem. 2012;133(2):585-591. PMID 25683437. DOI 10.1016/j.foodchem.2012.01.061. source
- Stability to light, heat and pH, and the effect of copper ionsReported that ergothioneine showed good light, thermal and acid-base stability, and that the presence of Cu2+ decreased the measured ergothioneine concentration.Liu X, Huang Y, Wang J, Zhou S. Int J Med Mushrooms. 2020;22(3):211-220. PMID 32479016. DOI 10.1615/IntJMedMushrooms.2020033625. source
