5-HTP (Griffonia simplicifolia seed extract) supplier specification
Nutrition BioTech is a bulk 5-HTP ingredient supplier and manufacturer, supplying Griffonia simplicifolia seed extract standardized to 20–98% 5-hydroxytryptophan (CAS 4350-09-8) by HPLC, for B2B nutraceutical formulation.
The wide standardization range is deliberate: a 20% Griffonia extract and a 98% 5-HTP grade behave very differently in label space, cost and blend design, so the assay grade is chosen per project rather than fixed. Every lot ships with a Certificate of Analysis stating botanical identity, HPLC assay and the assay basis.
| Ingredient name | 5-HTP, supplied as Griffonia simplicifolia seed extract standardized to 5-hydroxytryptophan |
|---|---|
| Synonyms | 5-HTP; L-5-hydroxytryptophan; oxitriptan; Griffonia seed extract; 5-hydroxy-L-tryptophan |
| CAS number | 4350-09-8 (L-5-hydroxytryptophan) |
| Machine-readable identifiers | InChIKey LDCYZAJDBXYCGN-VIFPVBQESA-N · PubChem CID 439280 |
| Molecular formula | C11H12N2O3 |
| Molecular weight | 220.22 g/mol |
| Assay / purity | Standardized from 20% to 98% 5-HTP by HPLC. The exact assay grade is specified per order and stated on the Certificate of Analysis for each lot. |
| Source / production route | Seed of Griffonia simplicifolia (Baill.), West and Central Africa; extraction and purification, with the solvent system stated on the specification sheet. |
| Appearance | Off-white to light beige or tan powder; shade depends on assay grade |
| Solubility | Slightly soluble in water and improves on warming; solubility of standardized extracts varies with assay grade. |
| Typical applications | Capsules, tablets, powder blends and stick packs for B2B nutraceutical manufacturing; commonly formulated alongside magnesium, L-theanine and GABA |
| Documentation | Download public specification sheet Public sheet prepared from the current product-page specification block; not a batch-specific Certificate of Analysis. Lot-specific COA and supporting records are available for qualified buyer review when appropriate. |
| Quality system | ISO 22000:2018, FSSC 22000, HACCP and GMP (certificates provided on request) |
| Supplier | Nutrition BioTech, 1601 W. Mission Blvd, Suite 103 (DL#29), Pomona, CA 91766, United States |
5-HTP 20-98%, Griffonia Seed Extract
Griffonia simplicifolia seed extract standardized to L-5-hydroxytryptophan (CAS 4350-09-8), available across a 20% to 98% assay range with the assay basis stated on each lot record.
Why brands ask for it
Nutrition BioTech supplies Griffonia simplicifolia seed extract standardized to L-5-hydroxytryptophan across a wide assay range. The material is used in capsules, tablets, sachets and powder blends. Buyers most often ask about the assay grade, the basis the assay is calculated on, the identity methods used, and the document set available for their market.
Formulation fit
Assay grade selection, label-space arithmetic at the chosen grade, particle behaviour in direct-compression versus capsule filling, and the document set your QA team needs. The strongest conversations start with target dosage form, serving size, active marker, and assay basis.
What makes the sample useful
- Standardized 5-HTP assay range
- Commonly co-formulated with magnesium, L-theanine and GABA
- Botanical seed origin with species-level identity documentation
Before the sample ships
- Confirm source, identity, and supporting documentation.
- Confirm active marker, assay method, and specification range.
- Match the material to the target capsule, tablet, sachet, gummy, or powder format.
- Request allergen, GMO, vegan, and source statements.
- Review serving-size logic and label-space fit.
File Package
Ask for the material files that match your market, dosage form, and internal review process.
Available documents may include: COA / Specification / Allergen Statement / GMO Statement / Vegan Statement / Heavy Metals / Microbial Limits / Manufacturing Flow Chart / Residual Solvent / Country of Origin / Composition Statement.
Regulatory & Facility Support
Regulatory review notes for the target market can be provided. Nutrition BioTech does not hold and does not claim any GRAS notification or NDI notification for this material.
Facility and quality system support follows the same certificate set named in the specification table: ISO 22000:2018, FSSC 22000, HACCP and GMP. The holding entity for each certificate is named on the certificate and confirmed under buyer review.
Specification FAQ
What is the CAS number for 5-HTP?
The CAS number for 5-HTP is 4350-09-8 (L-5-hydroxytryptophan). The molecular formula is C11H12N2O3 and the molecular weight is 220.22 g/mol. Machine-readable identifiers: InChIKey LDCYZAJDBXYCGN-VIFPVBQESA-N · PubChem CID 439280.
What purity is 5-HTP supplied at, and by which method?
Standardized from 20% to 98% 5-HTP by HPLC. The exact assay grade is specified per order and stated on the Certificate of Analysis for each lot.
Is 5-HTP soluble in water?
Slightly soluble in water and improves on warming; solubility of standardized extracts varies with assay grade.
How is 5-HTP produced and where does it come from?
Seed of Griffonia simplicifolia (Baill.), West and Central Africa; extraction and purification, with the solvent system stated on the specification sheet.
Published research context
5-Hydroxytryptophan (5-HTP) has a peer-reviewed analytical and botanical-identity literature. The studies below are provided as technical context for formulators evaluating this raw material. These entries summarise what the cited publications report. They are not claims about Nutrition BioTech material, and no health, therapeutic or cosmetic outcome is claimed or implied for any finished product. Buyers are responsible for the claims they make on their own labels and for confirming what is permitted in their market.
- Direct HPLC assay in Griffonia seeds Reported an HPLC method for the direct assay of 5-hydroxytryptophan in seeds of Griffonia simplicifolia using a single-step solvent extraction, and measured levels of up to 20.83% on a fresh-weight basis in seeds obtained in Ghana, the highest reported at that time. Lemaire PA, Adosraku RK. Phytochem Anal. 2002;13(6):333-337. PMID 12494751. DOI 10.1002/pca.659. source
- Botanical identity and chemical fingerprinting Characterised Griffonia simplicifolia seeds by HPLC-DAD-ESI-MS/MS for the nitrogen-containing compounds, GC-FID and GC-MS for the major fatty acids, and PCR-RFLP DNA fingerprinting for unequivocal identification of the plant; 5-HTP was the most representative compound, followed by lower percentages of the beta-carboline alkaloid derivative griffonine and other alkaloids. Vigliante I, Mannino G, Maffei ME. Molecules. 2019;24(6):1032. PMID 30875930. DOI 10.3390/molecules24061032. source
- Enantiomeric purity method for supplements Described a rapid electrokinetic chromatography method using 1.75% sulfated gamma-cyclodextrin in formate buffer at pH 2.2 for the enantiomeric determination of 5-hydroxytryptophan, noting that legal regulations do not allow the presence of the D-enantiomer in dietary supplements, so quality control requires chiral methodology; the method was applied to the analysis of a dietary supplement. Adámez-Rodríguez S, Marina ML, Castro-Puyana M. Analyst. 2026;151(4):1145-1152. PMID 41568872. DOI 10.1039/d5an01100k. source